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dapi reagent  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology dapi reagent
    Dapi Reagent, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+reagent/DAPI+Reagent/pm42869340-133-16-19
    Average 94 stars, based on 30 article reviews
    dapi reagent - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Dehydrocorybulbine blocks the Lyn-mediated MAPK pathway to promote anti-inflammatory polarization of microglia in mice after sevoflurane anesthesia.
    Article Snippet: Dehydrocorybulbine (DHCB) has demonstrated efficacy in alleviating thermally induced acute pain.. The present study sought to study the impact of DHCB on postoperative cognitive dysfunction (POCD).. Mice or mouse BV2 cells were exposed to sevoflurane for modeling.

    Article Title: Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress
    Article Snippet: After incubation with the primary antibodies and washing, all sections were incubated with secondary anti-rabbit AF647 (1:150, E-AB-1075, Elabscience Biotechnology, Houston, TX, USA) antibodies for 30 min. .. Afterwards, the sections were then incubated with DAPI reagent (E-IR-R103, Elabscience Biotechnology, USA) for 15 min to stain the nuclei. .. The sections were then washed with tap water, dried in ethanol, covered with permanent mounting media (Sub-X Mounting Medium; Leica Biosystems, Vista, CA, USA), and analyzed using an LSM 710 Carl Zeiss confocal microscope.

    Article Title: Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress.
    Article Snippet: After incubation with the primary antibodies and washing, all sections were incubated with secondary anti-rabbit AF647 (1:150, E-AB-1075, Elabscience Biotechnology, Houston, TX, USA) antibodies for 30 min. .. Afterwards, the sections were then incubated with DAPI reagent (E-IR-R103, Elabscience Biotechnology, USA) for 15 min to stain the nuclei. .. The sections were then washed with tap water, dried in ethanol, covered with permanent mounting media (Sub-X Mounting Medium; Leica Biosystems, Vista, CA, USA), and analyzed using an LSM 710 Carl Zeiss confocal microscope.

    Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection.

    Staining:

    Article Title: Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress
    Article Snippet: After incubation with the primary antibodies and washing, all sections were incubated with secondary anti-rabbit AF647 (1:150, E-AB-1075, Elabscience Biotechnology, Houston, TX, USA) antibodies for 30 min. .. Afterwards, the sections were then incubated with DAPI reagent (E-IR-R103, Elabscience Biotechnology, USA) for 15 min to stain the nuclei. .. The sections were then washed with tap water, dried in ethanol, covered with permanent mounting media (Sub-X Mounting Medium; Leica Biosystems, Vista, CA, USA), and analyzed using an LSM 710 Carl Zeiss confocal microscope.

    Article Title: MiR-218 Promotes Adriamycin-Induced H9C2 Apoptosis by Inhibiting Stress-Associated Endoplasmic Reticulum Protein 1
    Article Snippet: After washing the H9C2 cells twice with PBS, the TUNEL reaction mixture (R&D Systems, Minneapolis, MN, USA) was added dropwise to the slide and incubated for 1 hour at room temperature. .. Then, it was washed twice with PBS, and the nuclei were stained with DAPI reagent (Elabscience, Wuhan, China) in the dark room for 10 minutes, finally, observed under a fluorescence microscope (R&D Systems, Minneapolis, MN, USA). .. Luciferase plasmids (R&D Systems, Minneapolis, MN, USA) containing wild-type and mutant Serp1 mRNA 3′UTR were cotransfected with miR-218 mimics and miR-218 NC into 293 T cells (ATCC, Manassas, VA, USA), respectively.

    Article Title: Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress.
    Article Snippet: After incubation with the primary antibodies and washing, all sections were incubated with secondary anti-rabbit AF647 (1:150, E-AB-1075, Elabscience Biotechnology, Houston, TX, USA) antibodies for 30 min. .. Afterwards, the sections were then incubated with DAPI reagent (E-IR-R103, Elabscience Biotechnology, USA) for 15 min to stain the nuclei. .. The sections were then washed with tap water, dried in ethanol, covered with permanent mounting media (Sub-X Mounting Medium; Leica Biosystems, Vista, CA, USA), and analyzed using an LSM 710 Carl Zeiss confocal microscope.

    Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection.

    Fluorescence:

    Article Title: MiR-218 Promotes Adriamycin-Induced H9C2 Apoptosis by Inhibiting Stress-Associated Endoplasmic Reticulum Protein 1
    Article Snippet: After washing the H9C2 cells twice with PBS, the TUNEL reaction mixture (R&D Systems, Minneapolis, MN, USA) was added dropwise to the slide and incubated for 1 hour at room temperature. .. Then, it was washed twice with PBS, and the nuclei were stained with DAPI reagent (Elabscience, Wuhan, China) in the dark room for 10 minutes, finally, observed under a fluorescence microscope (R&D Systems, Minneapolis, MN, USA). .. Luciferase plasmids (R&D Systems, Minneapolis, MN, USA) containing wild-type and mutant Serp1 mRNA 3′UTR were cotransfected with miR-218 mimics and miR-218 NC into 293 T cells (ATCC, Manassas, VA, USA), respectively.

    Microscopy:

    Article Title: MiR-218 Promotes Adriamycin-Induced H9C2 Apoptosis by Inhibiting Stress-Associated Endoplasmic Reticulum Protein 1
    Article Snippet: After washing the H9C2 cells twice with PBS, the TUNEL reaction mixture (R&D Systems, Minneapolis, MN, USA) was added dropwise to the slide and incubated for 1 hour at room temperature. .. Then, it was washed twice with PBS, and the nuclei were stained with DAPI reagent (Elabscience, Wuhan, China) in the dark room for 10 minutes, finally, observed under a fluorescence microscope (R&D Systems, Minneapolis, MN, USA). .. Luciferase plasmids (R&D Systems, Minneapolis, MN, USA) containing wild-type and mutant Serp1 mRNA 3′UTR were cotransfected with miR-218 mimics and miR-218 NC into 293 T cells (ATCC, Manassas, VA, USA), respectively.



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